SCOPE Investigations into the immunological response of proteins is often masked by lipopolysaccharide (LPS) contamination. We report an optimized Triton X-114 (TX-114) based LPS extraction method for β-lactoglobulin (BLG) and soy protein extract suitable for cell-based immunological assays. METHODS AND RESULTS Optimization of an existing TX-114 based phase LPS extraction method resulted in >...

Flavonoids belong to a large group of natural polyphenol compounds and have a broad spectrum of pharmacological activities. Many natural flavonoids are characterized by extremely low solubility in aqueous media and body fluids. To improve the solubility of drugs, various methods were developed and are used in pharmaceutics: solubilization, preparation of solid disperse systems with soluble and ...

A pre-concentration and determination methodology for palladium in real samples at ultra-trace levels by 1-(2-pyridylazo)-2-naphthol (PAN) was developed. The analyte in the initial aqueous solution, acidified with HCl, was complexed with PAN and Triton X-114 was added as a surfactant. The surfactant-rich phase was diluted with concentrated HNO3 (65%, w/w) after phase separation. Then, the conce...

Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, Triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37 degrees C, whereupon two phases formed. The Triton X-114 phase, containing the endotoxin, was precipitated by centrifugation. The first cycle of phase separatio...

A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic p...

BACKGROUND The suitability of the strain Rhodococcus erythropolis ATCC 25544 grown in a two-liter fermentor as a source of cholesterol oxidase has been investigated. The strain produces both cell-linked and extracellular cholesterol oxidase in a high amount, that can be extracted, purified and concentrated by using the detergent Triton X-114. RESULTS A spray-dry method of preparation of the e...